Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CD47 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Polyclonal Rabbit anti-Human CD47 Antibody (IHC, IF)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Cynomolgus Monkey, Human |
| Format: | Liquid |
$148.00each
Catalog Number: LS-C817855-20
Catalog Number: LS-C817855
For bulk orders or custom formulations:
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- Description
- CD47 antibody LS-C817855 is an unconjugated rabbit polyclonal antibody to CD47 from human. It is reactive with human and crab-eating macaque. Validated for ELISA, ICC, IF and IHC.
- Application
- ELISA, ICC, IF, IHC-P
- Reactivity
- Cyno, Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD47 antigen | CD47 molecule | CD47 | Integrin associated protein | Integrin-associated protein | Leukocyte surface antigen CD47 | MER6 | Protein MER6 | Rh-related antigen | OA3 | CD47 glycoprotein | IAP
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- UniProt Number
- Q08722
- NCBI GenBank
- NM_001777 | NP_001768.1
- SwissProt
- Q08722
Target
- Target
- Human CD47
- Antigen Species
- Human
- Epitope
- Human CD47
- Gene Name
- CD47
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human CD47 protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS
