LSBio
Polyclonal Rabbit anti-Human CD274 / B7-H1 / PD-L1 Antibody (IHC, IF, WB)

Immunofluorescence analysis of HeLa cells using CD274 antibody. Blue: DAPI for nuclear staining.

Polyclonal Rabbit anti-Human CD274 / B7-H1 / PD-L1 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human CD274 / B7-H1 / PD-L1 antibodies →
$281.00each
Catalog Number: LS-C746930-20

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Overview

Description
PD-L1 antibody LS-C746930 is an unconjugated rabbit polyclonal antibody to PD-L1 (CD274 / B7-H1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 2 publications.
Application
IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Recommended Dilution: IF/ICC
1:20 - 1:100
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q9NZQ7
SwissProt
Q9NZQ7

Target

Target
Human CD274 / B7-H1 / PD-L1
Antigen Species
Hu
Gene Name
CD274

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-238 of human CD274 (NP_054862.1). FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
Immunogen Type
Fusion-P

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Concentration
1.66 mg/mL
Volume
20 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
anlotinib alters tumor immune microenvironment by downregulating PD-L1 expression on vascular endothelial cells. Shaochuan Liu , Tingting Qin , Zhujun Liu, Jing Wang, Yanan Jia, Yingfang Feng, Yuan Gao, Kai Li. Cell death & disease. 2020 May;11:309. PubMed: 32366856 PMC: PMC7198575

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