LSBio
Polyclonal Rabbit anti-Human CCND1 / Cyclin D1 Antibody (WB)

Western blot analysis is shown using Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10000 of HRP conjugated Go.

Polyclonal Rabbit anti-Human CCND1 / Cyclin D1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human CCND1 / Cyclin D1 antibodies →
$579.00each
Catalog Number: LS-C18809-100

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Overview

Description
Cyclin D1 antibody LS-C18809 is an unconjugated rabbit polyclonal antibody to human Cyclin D1 (CCND1). Validated for ELISA, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
B-cell lymphoma 1 protein | BCL-1 oncogene | B-cell CLL/lymphoma 1 | BCL-1 | Cyclin D1 | G1/S-specific cyclin-D1 | U21B31 | PRAD1 | BCL1 | CCND1 | D11S287E | PRAD1 oncogene
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P24385
SwissProt
P24385

Target

Target
Human CCND1 / Cyclin D1
Antigen Species
Human
Epitope
Antiserum will specifically react with a 40-45 kDa Cyclin D1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin D1 from other species may also occur.
Gene Name
CCND1

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.01% Sodium Azide
Concentration
85 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.

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