LSBio
Polyclonal Rabbit anti-Human CCNB1 / Cyclin B1 Antibody (IHC, IF, WB)

Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

Polyclonal Rabbit anti-Human CCNB1 / Cyclin B1 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human CCNB1 / Cyclin B1 antibodies →
$288.00each
Catalog Number: LS-C801555-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
Cyclin B1 antibody LS-C801555 is an unconjugated rabbit polyclonal antibody to Cyclin B1 (CCNB1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CCNB1 | CCNB | Cyclin B1 | G2/mitotic-specific cyclin-B1 | G2/mitotic-specific cyclin B1
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P14635
SwissProt
P14635

Target

Target
Human CCNB1 / Cyclin B1
Antigen Species
Human
Epitope
Cyclin B1 Antibody detects endogenous levels of total Cyclin B1.
Gene Name
CCNB1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthesized peptide derived from human Cyclin B1
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.