LSBio
Polyclonal Rabbit anti-Human CASP9 / Caspase 9 Antibody (IHC, WB)

Western blot of Caspase-9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 kD pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.

Polyclonal Rabbit anti-Human CASP9 / Caspase 9 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Dog, Human, Mouse, Rat
Format:Liquid
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$394.00each
Catalog Number: LS-C148248-50

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Overview

Description
Caspase 9 antibody LS-C148248 is an unconjugated rabbit polyclonal antibody to Caspase 9 (CASP9) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB. Cited in 1 publication.
Application
IHC, IHC-F, IHC-P, IP, WB
Reactivity
Can, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APAF3 | CASP9 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Recommended Dilution: IHC-P
1:1000 - 1:5000
Recommended Dilution: IP
1:50 - 1:200
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
P55211
SwissProt
P55211

Target

Target
Human CASP9 / Caspase 9
Antigen Species
Human
Epitope
This polyclonal antisera recognizes the proform of caspase-9 (~50 kD), and the large (~35 kD) and small (~15 kD) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kD.
Gene Name
CASP9

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Recombinant full length human Caspase-9 protein (pro-form).
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.05% sodium azide
Volume
50 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Caspase-9 acts as a regulator of necroptotic cell death10 Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Hungary. Tamás Molnár , Petra Pallagi , Bálint Tél , Róbert Király, Eszter Csoma, Viktória Jenei, Zsófia Varga , Péter Gogolák, Anne Odile Hueber, Zoltán Máté, Ferenc Erdélyi, Gábor Szabó, Aladár Pettkó-Szandtner, Attila Bácsi, László Virág, József Maléth , Gábor Koncz. The Breast : official journal of the European Society of Mastology. 2021 Nov;;288:6476-6491. PubMed: 33899329

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