LSBio
Polyclonal Rabbit anti-Human CA-VB / CA5B Antibody (Preservative Free, IF)

Immunofluorescence staining of CA5B in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CA5B polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

Polyclonal Rabbit anti-Human CA-VB / CA5B Antibody (Preservative Free, IF)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence
Reactivity:Human
Format:Liquid
See all Human CA-VB / CA5B antibodies →
$309.00each
Catalog Number: LS-C817795-100

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Overview

Description
CA5B antibody LS-C817795 is an unconjugated rabbit polyclonal antibody to human CA5B (CA-VB). Validated for ELISA, ICC and IF.
Application
ELISA, ICC, IF
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CA5B | CA-VB | Carbonate dehydratase VB | Carbonic anhydrase VB | Carbonic dehydratase
Recommended Dilution: ELISA
1:5000 - 1:10000
Recommended Dilution: IF/ICC
1:300 - 1:10000
UniProt Number
Q9Y2D0
NCBI GenBank
NM_007220 | NP_009151.1
SwissProt
Q9Y2D0

Target

Target
Human CA-VB / CA5B
Antigen Species
Human
Epitope
Human Carbonic Anhydrase VB
Gene Name
CA5B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant Human CA5B Protein
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.2 µm filtered solution in PBS
Volume
100 Microliter
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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