LSBio
Polyclonal Rabbit anti-Human BAX Antibody (aa43-61, IHC, WB)

Western blot of BAX in colon tumor cell lines using Polyclonal Antibody to Bax. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1

Polyclonal Rabbit anti-Human BAX Antibody (aa43-61, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$394.00each
Catalog Number: LS-C148224-50

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Overview

Description
BAX antibody LS-C148224 is an unconjugated rabbit polyclonal antibody to BAX (aa43-61) from human. It is reactive with human and monkey. Validated for IHC, IP and WB.
Application
IHC, IHC-F, IHC-P, IP, WB
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BAX | BCL2-associated X protein | Bcl2-L-4 | Apoptosis regulator BAX | Bcl-2-like protein 4 | BCL2L4
Recommended Dilution: IHC-P
1:1000 - 1:5000
Recommended Dilution: IP
1:50 - 1:200
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
Q07812
SwissProt
Q07812

Target

Target
Human BAX
Antigen Species
Human
Epitope
Bax.
Gene Name
BAX

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
A synthetic peptide corresponding to amino acids 43-61 (PELALDPVPQDASTKKLSE) of human Bax alpha. The immunogen sequence is 100% conserved in human Bax isoforms alpha (192 amino acids), beta (218amino acids), epsilon (164 amino acids), sigma (179 amino acids), and psi (173 amino acids). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset (100%); Galago, Panda, Cat (89%); Ferret, Sheep, Dog, Bovine, Horse, Pig (84%); Mouse, Rat (79%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.05% sodium azide
Volume
50 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.

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