Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size) in lane 1. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) incubated with the phosphopeptide immunogen. Primary Antibody : Aurora B (phospho T232) antibody diluted 1:500.
Polyclonal Rabbit anti-Human AURKB / Aurora-B Antibody (phospho-Thr232, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745289-25
Catalog Number: LS-C745289
For bulk orders or custom formulations:
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- Description
- Aurora-B antibody LS-C745289 is an unconjugated rabbit polyclonal antibody to Aurora-B (AURKB) (pThr232) from human. It is reactive with human and mouse. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AIRK2 | AIK2 | Aurora kinase B | Aurora kinase B-Sv1 | Aurora-related kinase 2 | Aurora/IPL1-related kinase 2 | ARK-2 | ARK2 | Aurkb-sv1 | Aurkb-sv2 | Aurora 1 | Aurora-B | AIM-1 | IPL1 | STK-1 | STK5 | PPP1R48 | AurB | AURKB | Aurora B | Aurora kinase B-Sv2 | Aurora-1 | Aurora1 | Serine/threonine kinase 12 | STK1 | STK12
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: WB
- 1:250 - 1:2000
- UniProt Number
- Q96GD4
- NCBI GenBank
- NM_004217 | NP_004208.2
- SwissProt
- Q96GD4
Target
- Target
- Human AURKB / Aurora-B
- Antigen Species
- Human
- Epitope
- Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.
- Gene Name
- AURKB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
