LSBio
Polyclonal Rabbit anti-Human ATP6V1F Antibody (IHC, IF)

Immunofluorescence staining of ATP6V1F in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ATP6V1F polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

Polyclonal Rabbit anti-Human ATP6V1F Antibody (IHC, IF)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
See all Human ATP6V1F antibodies →
$309.00each
Catalog Number: LS-C815948-100

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Overview

Description
ATP6V1F antibody LS-C815948 is an unconjugated rabbit polyclonal antibody to human ATP6V1F. Validated for ICC, IF and IHC.
Application
ICC, IF, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ATPase, vacuolar, 14 kD | ATP6V1F | V-ATPase subunit F | Vacuolar proton pump F subunit | Vma7 | V-ATPase F subunit | ATP6S14 | Vacuolar proton pump subunit F | V-ATPase 14 kDa subunit | V-type proton ATPase subunit F | VATF
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:50 - 1:200
UniProt Number
Q16864
SwissProt
Q16864

Target

Target
Human ATP6V1F
Antigen Species
Human
Epitope
Human ATP6V1F
Gene Name
ATP6V1F

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
E. coli-derived Human ATP6V1F fragment
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.0, 0.03% Proclin 300
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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