LSBio
Polyclonal Rabbit anti-Human ATP1A1 Antibody (phospho-Ser23, IF, WB)

Staining PC-12 cells treated with TPA cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

Polyclonal Rabbit anti-Human ATP1A1 Antibody (phospho-Ser23, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human ATP1A1 antibodies →
$288.00each
Catalog Number: LS-C800970-100

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Overview

Description
ATP1A1 antibody LS-C800970 is an unconjugated rabbit polyclonal antibody to ATP1A1 (pSer23) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Application
ELISA, IF, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ATP1A1 | Na+, K+ ATPase alpha subunit | Na,K-ATPase alpha-1 subunit | Na+/K+ ATPase 1 | Sodium pump 1 | Sodium pump subunit alpha-1
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P05023
NCBI GenBank
NM_000701 | NP_000692.2
SwissProt
P05023

Target

Target
Human ATP1A1
Antigen Species
Human
Epitope
Phospho-ATP1 alpha1/Na+K+ ATPase1 (Ser23) Antibody detects endogenous levels of ATP1 alpha1/Na+K+ ATPase1 only when phosphorylated at Serine 23.
Gene Name
ATP1A1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthesized peptide derived from human ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Serine 23.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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