LSBio
Polyclonal Rabbit anti-Human ARRB1 + ARRB2 Antibody (IHC, WB)

IHC (P) using pan Arrestin Antibody, at a dilution of 1:500.

Polyclonal Rabbit anti-Human ARRB1 + ARRB2 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Bovine, Hamster, Human, Mouse, Non-Human Primate, Rat, Xenopus, Zebrafish
Format:Liquid
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$470.00each
Catalog Number: LS-B15546-50

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Overview

Description
ARRB1 + ARRB2 antibody LS-B15546 is an unconjugated rabbit polyclonal antibody to ARRB1 + ARRB2 from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB. Cited in 1 publication.
Application
IHC, IHC-P, IP, WB
Reactivity
Bov, Ham, Hu, Ms, NHP, Rt, Xen, Zf
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
3 µg/ml

Target

Target
ARRB1 + ARRB2
Antigen Species
Human
Specificity
The antibody detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin. By Western blot, this antibody detects ~49 kD and ~47 kD proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.
Epitope
The antibody detects recombinant rat and human beta-arrestin and beta-arrestin2. This antibody does not detect visual or cone arrestin. By Western blot, this antibody detects ~49 kD and ~47 kD proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Synthetic peptide corresponding to aa384-397 (DDIVFEDFARLRLK). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Hamster, Panda, Xenopus, Zebrafish (100%); Drosophila, Mosquito, Beetle (93%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Acute ethanol exposure reduces serotonin receptor 1A internalization by increasing ubiquitination and degradation of ß-arrestin2. Deborah J Luessen, Haiguo Sun, Molly M McGinnis, Michael Hagstrom, Glen Marrs, Brian A McCool, Rong Chen. The Journal of biological chemistry. 2019 September;294:14068-14080. PubMed: 31366729 PMC: PMC6755807

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