LSBio
Polyclonal Rabbit anti-Human ARFGAP3 Antibody (IF, WB)

Immunofluorescence Microscopy of rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent sinal.

Polyclonal Rabbit anti-Human ARFGAP3 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$311.00each
Catalog Number: LS-C744614-25

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Overview

Description
ARFGAP3 antibody LS-C744614 is an unconjugated rabbit polyclonal antibody to human ARFGAP3. Validated for ELISA, IF, IP and WB.
Application
ELISA, IF, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ARF GAP 3 | ARFGAP3
Recommended Dilution: ELISA
1:5000
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: WB
1:1000
UniProt Number
Q9NP61
NCBI GenBank
NM_014570 | NP_055385.3
SwissProt
Q9NP61

Target

Target
Human ARFGAP3
Antigen Species
Human
Epitope
The antibody detects ArfGAP3 in cell lysates. A BLAST analysis was used to suggest cross reactivity with human, monkey, and orangutan for ArfGAP3. Cross-reactivity with AfGAP3 from other sources have not been determined.
Gene Name
ARFGAP3

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
70 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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