LSBio
Polyclonal Rabbit anti-Human APG5 / ATG5 Antibody (IF, WB)

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite.

Polyclonal Rabbit anti-Human APG5 / ATG5 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Western Blot
Reactivity:Human
Format:Liquid
See all Human APG5 / ATG5 antibodies →
$451.00each
Catalog Number: LS-C775310-100

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Overview

Description
ATG5 antibody LS-C775310 is an unconjugated rabbit polyclonal antibody to human ATG5 (APG5). Validated for IF and WB.
Application
IF, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | ATG5 | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: WB
1:1000
UniProt Number
Q9H1Y0
SwissProt
Q9H1Y0

Target

Target
Human APG5 / ATG5
Antigen Species
Hu
Epitope
Predicted molecular weight at ~32.4 kDa. Observed molecular weights at ~48-60 kDa based on ATG12-ATG5 heterodimer.
Gene Name
ATG5

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide from the mid-protein of human ATG5
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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