LSBio
Polyclonal Rabbit anti-Human AKT1 Antibody (IHC, WB)

Western Blot of Anti-AKT (RABBIT) Antibody. Lane 1: SuperSignal MW markers. Lane 2: rAKT1. Lane 3: rAKT2. Lane 4: rAKT3. Load: 50 ng per lane. Primary antibody: Anti- AKT (RABBIT) Antibody at 1:1,000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070), 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3.

Polyclonal Rabbit anti-Human AKT1 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$311.00each
Catalog Number: LS-C745007-25

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Overview

Description
AKT1 antibody LS-C745007 is an unconjugated rabbit polyclonal antibody to AKT1 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Application
ELISA, FC, IHC, WB
Reactivity
Hu, Ms, Rt
Predicted Reactivity
Ck
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
P31749
NCBI GenBank
NM_005163, NP_005154.2
SwissProt
P31749

Target

Target
Human AKT1
Antigen Species
Hu
Epitope
Reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
Gene Name
AKT1

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.5 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.