Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western blot of anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag-tagged human ABCB6 protein at 104 kD and a truncated form of the protein at 79 kD (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A Lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag antibody as a positive control. The membrane was probe.
Polyclonal Rabbit anti-Human ABCB6 Antibody (WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C19004-100
Catalog Number: LS-C19004
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- Description
- ABCB6 antibody LS-C19004 is an unconjugated rabbit polyclonal antibody to ABCB6 from human. It is reactive with human and dog. Validated for ELISA and WB. Cited in 1 publication.
- Application
- ELISA, IHC, WB
- Predicted Reactivity
- Dog, Human, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ABC14 | ABC | ABCB6 | EST45597 | LAN | MTABC3 | PrP | P-glycoprotein-related protein | MCOPCB7 | Mt-ABC transporter 3 | Umat
- Recommended Dilution: ELISA
- 1:40000 - 1:130000
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- Q9NP58
- NCBI GenBank
- AAH00559.1
- SwissProt
- Q9NP58
Target
- Target
- Human ABCB6
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross reactivity with ABCB6 protein from human and dog based on 100% homology with the immunizing sequence. Expect partial reactivity with ABCB6 from mouse and rat sources based on partial (~93%, 15/16) sequence homology. Reactivity against homologues from other sources is not known.
- Gene Name
- ABCB6
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 430-465 of human ABCB6 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Classification of subcellular location by comparative proteomic analysis of native and density-shifted lysosomes. Della Valle MC, Sleat DE, Zheng H, Moore DF, Jadot M, Lobel P. Molecular & cellular proteomics : MCP. 2011;10:M110.006403. PubMed: 21252268 PMC: PMC3069348
