LSBio
Polyclonal Rabbit anti-Human ABCB5 Antibody (Internal, IHC, WB)

IHC - ABCB5 Antibody. Immunohistochemistry of ABCB5 antibody tissue: human liver Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: ABCB5 1:200 Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Polyclonal Rabbit anti-Human ABCB5 Antibody (Internal, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human ABCB5 antibodies →
$579.00each
Catalog Number: LS-C154236-100

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Overview

Description
ABCB5 antibody LS-C154236 is an unconjugated rabbit polyclonal antibody to human ABCB5 (Internal). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ABCB5alpha | ABCB5 P-gp | ABCB5beta | ABCB5 | ATP-binding cassette protein | EST422562 | P-glycoprotein ABCB5
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC-P
1:200
Recommended Dilution: WB
1:20000
UniProt Number
Q2M3G0
SwissProt
Q2M3G0

Target

Target
Human ABCB5
Antigen Species
Human
Epitope
This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources. Cross-reactivity with ABCB5 from other sources has not been determined.
Gene Name
ABCB5

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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