LSBio
Polyclonal Rabbit anti-E. coli PHOA Antibody (Liquid, Biotin, WB)

Western Blot of rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Streptavidin (S000-03) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).

Polyclonal Rabbit anti-E. coli PHOA Antibody (Liquid, Biotin, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:E. coli
Format:Liquid
See all E. coli PHOA antibodies →
$311.00each
Catalog Number: LS-C745121-25

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Overview

Description
PHOA antibody LS-C745121 is a biotin-conjugated rabbit polyclonal antibody to e. coli PHOA. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
E.col
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Bacterial alkaline phosphatase | JW0374 | PHOA | PsiA | ECK0378
Recommended Dilution: ELISA
1:4000 - 1:20000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P00634
SwissProt
P00634

Target

Target
E. coli PHOA
Antigen Species
E.col
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.
Gene Name
phoA

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
E.col

Immunogen

Immunogen
Alkaline Phosphatase [E.coli]

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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