LSBio
Polyclonal Rabbit anti-E. coli E. coli LMW Proteins Antibody (WB)

2D Western Blot of anti-E.coli HCP antibody. Load: 35 ug Total HCP. pH: 5-8. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4 degrees C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT.

Polyclonal Rabbit anti-E. coli E. coli LMW Proteins Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:E. coli
Format:Liquid
See all E. coli LMW Proteins antibodies →
$579.00each
Catalog Number: LS-C209182-100

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Overview

Description
E. coli LMW Proteins antibody LS-C209182 is an unconjugated rabbit polyclonal antibody to e. coli E. coli LMW Proteins. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
E.col
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:1000 - 1:2000
Recommended Dilution: WB
1:500 - 1:1000

Target

Target
E. coli LMW Proteins
Antigen Species
E. coli
Epitope
The anti-sera reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Anti-Host Cell Proteins Antibody was prepared blending antibodies that have been separately developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
77.5 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.