LSBio
Polyclonal Rabbit anti-E. coli E. coli / Escherichia coli Antibody (Biotin, WB)

2D Western Blot of anti-E.coli Host Cell Protein antibody. Load: 35 ug Total HCP. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4 degrees C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti-E. coli E. coli / Escherichia coli Antibody (Biotin, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:E. coli
Format:Lyophilized
See all E. coli / Escherichia coli antibodies →
$571.00each
Catalog Number: LS-C209221-100

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Overview

Description
Escherichia coli antibody LS-C209221 is a biotin-conjugated rabbit polyclonal antibody to e. coli Escherichia coli (E. coli). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
E.col
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:1000 - 1:2000
Recommended Dilution: WB
1:500 - 1:1000

Target

Target
E. coli / Escherichia coli
Antigen Species
E. coli

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.