LSBio
Polyclonal Goat Biotin Antibody (FITC, IF, WB)

Western blot of Fluorescein conjugated Goat F(ab')2 Anti-Biotin secondary antibody. Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.

Polyclonal Goat Biotin Antibody (FITC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Flow Cytometry, Immunofluorescence, Western Blot
Format:Lyophilized
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$706.00each
Catalog Number: LS-C154332-1

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Overview

Description
Biotin antibody LS-C154332 is an FITC-conjugated goat polyclonal antibody to Biotin. Validated for Flow, IF and WB.
Application
FC, IF, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: FC
1:500 - 1:2500
Recommended Dilution: IF/ICC
1:1000 - 1:5000

Target

Target
Biotin
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein Biotinylated IgG and Biotinylated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.