LSBio
Polyclonal Goat Anti-Rat IgG Secondary Antibody (IHC, WB)

Western blot of Goat anti-Rat IgG Antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat antibody at 1000 overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:20000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rat IgG. Other band(s): None. This image was taken for the unmodified form of this product. Other forms have not been tested.

Polyclonal Goat Anti-Rat IgG Secondary Antibody (IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rat
Format:Liquid
See all Rat IgG antibodies →
$411.00each
Catalog Number: LS-C154377-1

For bulk orders or custom formulations:

Request a Quote

Overview

Description
Rat IgG antibody LS-C154377 is an unconjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Rat IgG
Antigen Species
Rat
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Rat IgG was produced by repeated immunization with Rat IgG whole molecule in goat.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.