Polyclonal Goat anti-Goat Biotin Antibody (FITC, IF)
Catalog Number: LS-C758060-1
1 mg / $322.00
Antibody: Goat Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence
Reactivity: Goat
Format: Liquid
Western blot of Goat anti-Rat IgG Antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat antibody at 1000 overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:20000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rat IgG. Other band(s): None. This image was taken for the unmodified form of this product. Other forms have not been tested.
Polyclonal Goat Anti-Rat IgG Secondary Antibody (IHC, WB)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Rat |
| Format: | Liquid |
$411.00each
Catalog Number: LS-C154377-1
Catalog Number: LS-C154377
For bulk orders or custom formulations:
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- Description
- Rat IgG antibody LS-C154377 is an unconjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
Target
- Target
- Rat IgG
- Target Heavy Chain
- IgG
- Antigen Species
- Rt
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.
Antibody
- Host Species
- Gt
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-Rat IgG was produced by repeated immunization with Rat IgG whole molecule in goat.
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
