Polyclonal Goat anti-Goat Biotin Antibody (FITC, IF)
Catalog Number: LS-C758060-1
1 mg / $322.00
Antibody: Goat Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence
Reactivity: Goat
Format: Liquid
Western Blot of Anti-Rat IgG (H&L) (GOAT) antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule Lane 2: Rat IgG F(c) Fragment Lane 3: Rat IgG Fab Fragment Lane 4: Rat IgM Whole Molecule Lane 5: Rat Serum All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Polyclonal Goat Anti-Rat IgG Secondary Antibody (Atto 488, IF, WB)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Immunofluorescence, Other, Western Blot |
| Reactivity: | Rat |
| Format: | Lyophilized |
$317.00each
Catalog Number: LS-C745601-100
Catalog Number: LS-C745601
For bulk orders or custom formulations:
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- Description
- Rat IgG antibody LS-C745601 is an Atto 488-conjugated goat polyclonal antibody. Validated for FLISA, IF and WB.
- Application
- IF, Other, WB
- Reactivity
- Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: IF/ICC
- 1:5000
- Recommended Dilution: Other
- FLISA: 1:20000
- Recommended Dilution: WB
- 1:10000
Target
- Target
- Rat IgG
- Target Heavy Chain
- IgG
- Antigen Species
- Rt
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.
Antibody
- Host Species
- Gt
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Rat IgG whole molecule
- Immunogen Type
- Native
Format
- Conjugate/Label
- Atto 488
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
