HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4?. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.