LSBio
Polyclonal Goat Anti-Rabbit IgG Fc Secondary Antibody (Biotin, IHC, WB)

Western Blot - F(ab)2 Rabbit IgG F(c) Antibody Biotin Conjugated. Western blot of Goat anti-F(ab)2 Rabbit IgG F(c) Antibody Biotin Conjugated Lane 1: Rabbit Fc Load: 100 ng per lane Primary antibody: F(ab)2 Rabbit IgG F(c) Antibody Biotin Conjugated at 1:1000 for overnight at 4C Secondary antibody: HRP Streptavidin at 1:40000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 28 kD, 28 kD.

Polyclonal Goat Anti-Rabbit IgG Fc Secondary Antibody (Biotin, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$400.00each
Catalog Number: LS-C61483-500

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Overview

Description
Rabbit IgG Fc antibody LS-C61483 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Rb
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Rabbit IgG Fc
Target Heavy Chain
IgG
Antigen Species
Rb
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab).

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG F(c) fragment
Immunogen Type
Native

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.