Phycoerythrin Goat Anti-Mouse IgG (H&L) Antibody - Dot Blot. Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200 ng was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto (B501-0500) 1 Hour at 20?, Anti-Mouse IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated Min X By Bv, Ch, Gt, GP, Ham, Hs, Hu, Rb, Rt, & Sh Serum Proteins (p/n Anti-MOUSE IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Hs Ham Hu Rb Rt &Sh Serum Proteins) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. This image was taken for the unconjugated form of this product. Other forms have not been tested.