LSBio
Polyclonal Goat Anti-Mouse IgG Secondary Antibody (IHC, WB)

ELISA results of purified Goat anti-Mouse IgG Antibody Min X human serum tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG red line) Human IgG (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Gt-anti-Donkey HRP conjugate and TMB-1000 substrate.

Polyclonal Goat Anti-Mouse IgG Secondary Antibody (IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse IgG antibodies →
$337.00each
Catalog Number: LS-C60700-2

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Overview

Description
Mouse IgG antibody LS-C60700 is an unconjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:100000 - 1:500000
Recommended Dilution: IHC
1:5000 - 1:25000
Recommended Dilution: WB
1:10000 - 1:50000

Target

Target
Mouse IgG
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity against Human IgG.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2.2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Stable for several weeks at 4°C undiluted. Dilute only prior to immediate use. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.