LSBio
Polyclonal Goat Anti-Mouse IgG Secondary Antibody (FITC, IHC, IF, WB)

Fluorescein Goat Anti-Mouse IgG (H&L) Antibody - Dot Blot. Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200 ng was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto (B501-0500) 1 Hour at 20?, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (p/n Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins)) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat Anti-Mouse IgG Secondary Antibody (FITC, IHC, IF, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Other, Western Blot
Reactivity:Mouse
Format:Lyophilized
See all Mouse IgG antibodies →
$400.00each
Catalog Number: LS-C60676-1

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Overview

Description
Mouse IgG antibody LS-C60676 is an FITC-conjugated goat polyclonal antibody. Validated for DB, FLISA, Flow, IF, IHC and WB.
Application
DB, FC, IF, IHC, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: FC
1:500 - 1:2500
Recommended Dilution: IF/ICC
1:1000 - 1:5000
Recommended Dilution: Other
FLISA: 1:10000 - 1:50000

Target

Target
Mouse IgG
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.