LSBio
Polyclonal Goat Anti-Mouse IgG Fc Secondary Antibody (IHC, WB)

ELISA results of Goat anti-Mouse IgG F(c) Antibody tested against purified Mouse IgG F(c) protein. Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c) The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) and TMB substrate

Polyclonal Goat Anti-Mouse IgG Fc Secondary Antibody (IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse IgG Fc antibodies →
$319.00each
Catalog Number: LS-C60697-2

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Overview

Description
Mouse IgG Fc antibody LS-C60697 is an unconjugated goat polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Application
ELISA, IHC, IP, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:50000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Mouse IgG Fc
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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