LSBio
Polyclonal Goat Anti-Human IgG Fc Secondary Antibody (Biotin, IHC, WB)

Western Blot - F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed. Western Blot of Goat anti-F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed Lane 1: Human Fc Load: 100 ng per lane Primary Antibody: F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed Size: 28 kD/28 kD.

Polyclonal Goat Anti-Human IgG Fc Secondary Antibody (Biotin, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
See all Human IgG Fc antibodies →
$426.00each
Catalog Number: LS-C61384-1

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Overview

Description
Human IgG Fc antibody LS-C61384 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IB, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:125000 - 1:175000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Human IgG Fc
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG F(c) fragment.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at 20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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