LSBio
Polyclonal Goat anti-Horseradish HRP / Horseradish Peroxidase Antibody (WB)

Anti-Peroxidase Polyclonal Antibody-Western blot. Goat anti-Peroxidase antibody was used to detect horse radish peroxidase under reducing and non-reducing conditions. Reduced samples of purified horse radish peroxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti-Peroxidase antibody (1:5K in MB-0070, overnight at 4C). Primary antibody was detected with Dylight 649 conjugated Donkey anti-Goat (1:10K 1.5 hr RT in MB-070 and imaged on the Bio-Rad VersaDoc imaging system.

Polyclonal Goat anti-Horseradish HRP / Horseradish Peroxidase Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Plant
Format:Lyophilized
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$426.00each
Catalog Number: LS-C153963-2

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Overview

Description
Horseradish Peroxidase antibody LS-C153963 is an unconjugated goat polyclonal antibody to horseradish Horseradish Peroxidase (HRP). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Plnt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:9000 - 1:40000
Recommended Dilution: WB
1:900 - 1:4000

Target

Target
Horseradish HRP / Horseradish Peroxidase
Antigen Species
Plant
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Antibody

Host Species
Goat
Clonality
Polyclonal

Immunogen

Immunogen
Peroxidase [Horseradish].

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl
Concentration
45 mg/mL
Volume
2 Milliliter
Preservative
No
Purification Method
Other

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.01 M Sodium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.