LSBio
Polyclonal Goat Anti-Horse IgG Fab'2 Secondary Antibody (HRP, IHC, WB)

ELISA results of purified Polyclonal Goat Anti-Horse IgG F(ab')2 Antibody Peroxidase Conjugated tested against Horse IgG F(ab')2 (red) and Horse IgG (green). Each well was coated in duplicate with 1.0µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate

Polyclonal Goat Anti-Horse IgG Fab'2 Secondary Antibody (HRP, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Horse
Format:Lyophilized
See all Horse IgG Fab'2 antibodies →
$384.00each
Catalog Number: LS-C59914-2

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Overview

Description
Horse IgG Fab'2 antibody LS-C59914 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Hr
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:50000 - 1:150000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Horse IgG Fab'2
Antigen Species
Horse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum. No reaction was observed against Horse IgG F(c).

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Horse IgG was produced by repeated immunization with horse IgG F(ab')2 fragment in goat.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at 20°C. Avoid freeze-thaw cycles, store undiluted. Expiration date is 1 year from date of restoration.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.