LSBio
Polyclonal Donkey Anti-Sheep IgG Secondary Antibody (Biotin, IHC, WB)

ELISA results of purified Donkey anti-Sheep IgG Biotin Conjugated Antibody tested against Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB substrate.

Polyclonal Donkey Anti-Sheep IgG Secondary Antibody (Biotin, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Other, Western Blot
Reactivity:Sheep
Format:Lyophilized
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$329.00each
Catalog Number: LS-C61150-2

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Overview

Description
Sheep IgG antibody LS-C61150 is a biotin-conjugated donkey polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Application
ELISA, IB, IHC, Other, WB
Reactivity
Sh
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Sheep IgG
Target Heavy Chain
IgG
Antigen Species
Sh
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, sheep IgG and sheep serum.

Antibody

Host Species
Dk
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Sheep IgG whole molecule was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Immunogen Type
Native

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Prior to reconstitution store at 4°C. After reconstitution store at -20°C. Store undiluted. Avoid freeze/thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.