Donkey Serum Antibody
Catalog Number: LS-C835454-2
2 mL / $283.00
Antibody: Rabbit Polyclonal
Applications: Other
Reactivity: Donkey
Format: Liquid
ELISA results of purified Donkey Anti-Mouse IgG Alkaline Phosphatase Conjugated Antibody tested against purified Mouse IgG. (Blue Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer and TMB substrate
Polyclonal Donkey Anti-Mouse IgG Secondary Antibody (AP, IHC, WB)
Secondary AntibodyLSBio Guarantee
| Antibody: | Donkey Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$448.00each
Catalog Number: LS-C60844-1
Catalog Number: LS-C60844
For bulk orders or custom formulations:
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- Description
- Mouse IgG antibody LS-C60844 is an AP-conjugated donkey polyclonal antibody. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:2000 - 1:20000
- Recommended Dilution: IHC
- 1:500 - 1:2500
- Recommended Dilution: WB
- 1:1000 - 1:5000
Target
- Target
- Mouse IgG
- Antigen Species
- Mouse
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Antibody
- Host Species
- Donkey
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Mouse IgG whole molecule
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Alkaline Phosphatase
- Format
- Liquid
- Formulation
- 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C. Do not freeze.
- Recommended Storage Buffer
- 0.05 M Tris-Chloride|0.15 M NaCl
