LSBio
Polyclonal Donkey Anti-Goat IgG Secondary Antibody (Biotin, IHC, WB)

ELISA results of purified Donkey anti-Goat IgG antibody Biotin conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate

Polyclonal Donkey Anti-Goat IgG Secondary Antibody (Biotin, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Donkey Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Other, Western Blot
Reactivity:Goat
Format:Lyophilized
See all Goat IgG antibodies →
$334.00each
Catalog Number: LS-C60370-2

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Overview

Description
Goat IgG antibody LS-C60370 is a biotin-conjugated donkey polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IB, IHC, Other, WB
Reactivity
Gt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:200000 - 1:250000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Goat IgG
Antigen Species
Goat
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, goat IgG and goat serum.

Antibody

Host Species
Donkey
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.