LSBio
Polyclonal Chicken anti-Human LINE-1 Antibody (WB)

Western blot using the IgY fraction of anti-L1/ORF2 antibody shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).

Polyclonal Chicken anti-Human LINE-1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Chicken Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human LINE-1 antibodies →
$311.00each
Catalog Number: LS-C745202-25

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Overview

Description
LINE-1 antibody LS-C745202 is an unconjugated chicken polyclonal antibody to LINE-1 from human. It is reactive with human and chimpanzee. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
NHP
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:10000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Human LINE-1
Antigen Species
Hu
Epitope
A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.

Antibody

Host Species
Ck
Clonality
Polyclonal
Isotype
IgY

Immunogen

Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.