Western blot analysis of extracts of various cell lines, using POLR2E antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

POLR2E Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C331669-20

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Overview

Description: POLR2E antibody LS-C331669 is an unconjugated rabbit polyclonal antibody to POLR2E from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
Application: IF, IHC, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HsRPB5 | POLR2E | RPABC1 | RPB5 | XAP4 | HRPB25 | RPB5 homolog
Recommended Dilution: IF/ICC: 1:50 - 1:200
Recommended Dilution: IHC: 1:50 - 1:100
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P19388
NCBI GenBank: NM_002695 | NP_002686.2
SwissProt: P19388

Target

Target: Human POLR2E
Antigen Species: Human
Epitope: Human POLR2E
Gene Name: POLR2E

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2). MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)