LSBio
POLR2A / RNA polymerase II Antibody (phospho-Ser2/Ser5)

POLR2A / RNA polymerase II Antibody (phospho-Ser2/Ser5)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human POLR2A / RNA polymerase II antibodies →
$703.00each
Catalog Number: LS-C17711-100

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Overview

Description
RNA polymerase II antibody LS-C17711 is an unconjugated rabbit polyclonal antibody to RNA polymerase II (POLR2A) (pSer2/Ser5) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Application
IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | POLR2A | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Recommended Dilution: IHC-P
1:25
Recommended Dilution: WB
1:1000
UniProt Number
P24928
SwissProt
P24928

Target

Target
Human POLR2A / RNA polymerase II
Antigen Species
Human
Epitope
Detects endogenous levels of Rpb1 only when the carboxy-terminal domain heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at serine 2/5. Species cross-reactivity: Human, mouse, and rat. Species sequence homology: Hamster.
Gene Name
POLR2A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Volume
100 Microliter
Preservative
No
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

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