Western blot testing of human HepG2, K562, and SK-BR-3 cell lysate with phospho-RNA polymerase II antibody (clone CTD 8A7). Routinely observed molecular weight: 200-250 kDa.
POLR2A / RNA polymerase II Antibody (clone CTD 8A7)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, S. cerevisiae |
| Format: | Liquid |
$379.00each
Catalog Number: LS-C782734-20
Catalog Number: LS-C782734
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- Description
- RNA polymerase II antibody LS-C782734 is an unconjugated mouse monoclonal antibody to RNA polymerase II (POLR2A) from human. It is reactive with human and saccharomyces cerevisiae. Validated for IF, IHC and WB.
- Application
- IF, IHC-P, WB
- Reactivity
- Hu, S.cer
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | POLR2A | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
- Recommended Dilution: IF/ICC
- 1 - 2 µg/ml
- Recommended Dilution: IHC-P
- 1 - 2 µg/ml
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- P24928
- NCBI GenBank
- NM_000937 | NP_000928.1
- SwissProt
- P24928
Target
- Target
- Human POLR2A / RNA polymerase II
- Antigen Species
- Human
- Gene Name
- POLR2A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- CTD 8A7
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- The immunogen for this phospho-RNA polymerase II antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
- Concentration
- 0.2 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 2-8°C.
- Recommended Storage Buffer
- 1X PBS