Flow Cytometry analysis of HEPG2 cells using anti-Human PNP antibody. Overlay histogram showing HEPG2 cells stained with anti-Human PNP antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human PNP Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PNP / Nucleoside Phosphorylase Antibody (DY488)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry |
| Reactivity: | Human |
| Format: | Liquid |
$583.00each
Catalog Number: LS-C756444-139
Catalog Number: LS-C756444
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- Description
- Nucleoside Phosphorylase antibody LS-C756444 is a DY488-conjugated rabbit polyclonal antibody to human Nucleoside Phosphorylase (PNP). Validated for Flow.
- Application
- FC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Inosine phosphorylase | NP | Nucleoside phosphorylase | PNP | PUNP | PRO1837
- Recommended Dilution: FC
- 1 - 3 µg/10E6 cells
- UniProt Number
- P00491
- NCBI GenBank
- NM_000270 | NP_000261.2
- SwissProt
- P00491
Target
- Target
- Human PNP / Nucleoside Phosphorylase
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- PNP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human PNP (161-189 aa AMSDAYDRTMRQRALSTWKQMGEQRELQE), different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- DyLight 488
- Format
- Liquid
- Formulation
- 0.2% Na2HPO40.9% NaCl, 0.02% sodium azide, 50% glycerol
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C. Do not freeze. Protect from light.
- Recommended Storage Buffer
- NaCl