LSBio
PMEL / SILV / gp100 Antibody (clone OTI7E3)

HEK293T cells transfected with either pCMV6-ENTRY SILV (Red) or empty vector control plasmid (Blue) were immunostained with anti-SILV mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.

PMEL / SILV / gp100 Antibody (clone OTI7E3)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general)
Reactivity:Human
Format:Liquid
See all Human PMEL / SILV / gp100 antibodies →
$443.00each
Catalog Number: LS-C114528-100

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Overview

Description
Gp100 antibody LS-C114528 is an unconjugated mouse monoclonal antibody to human gp100 (PMEL / SILV). Validated for Flow and IHC. Cited in 1 publication.
Application
FC, IHC
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
gp100 | Melanocyte protein PMEL | Me20m/me20s | p100 | PMEL17 | Premelanosome protein | SIL | Silver homolog (mouse) | Silver, mouse, homolog of | Silver locus protein homolog | ME20-M | Me20-m/me20-s | ME20M | Melanocyte protein mel 17 | Melanocyte protein Pmel 17 | PMEL | Silver (mouse homolog) like | D12S53E | ME20 | Melanosomal matrix protein17 | SILV
Recommended Dilution: FC
1:100
Recommended Dilution: IHC
1:150
UniProt Number
P40967
SwissProt
P40967

Target

Target
Human PMEL / SILV / gp100
Antigen Species
Human
Epitope
Human PMEL / SILV / gp100
Gene Name
PMEL

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OTI7E3
Isotype
IgG2b

Immunogen

Immunogen
Full length human recombinant protein of human SILV (NP_008859) produced in HEK293T cell.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Landscape mapping of shared antigenic epitopes and their cognate TCRs of tumor-infiltrating T lymphocytes in melanoma. Kenji Murata , Munehide Nakatsugawa , Muhammed A Rahman, Linh T Nguyen, Douglas G Millar, David T Mulder, Kenji Sugata, Hiroshi Saijo, Yukiko Matsunaga, Yuki Kagoya, Tingxi Guo, Mark Anczurowski, Chung-Hsi Wang, Brian D Burt, Dalam Ly, Kayoko Saso, Alexandra Easson, David P Goldstein, Michael Reedijk, Danny Ghazarian, Trevor J Pugh, Marcus O Butler, Tak W Mak, Pamela S Ohashi, Naoto Hirano. eLife. 2020 Apr;9:e53244. PubMed: 32314731 PMC: PMC7234812

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