Western Blot of rabbit Anti-PKM2 antibody. Lane 1 and 2: iPSC lysate. Lane 3 and 4: myeloid lysate (iPSC derived). Load: 100 µg per lane. Primary antibody: PKM2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Odyssey blocking buffer for 120 min at RT. Predicted/Observed size: 57 kda for PKM2. Other band(s): PKM2 splice variants and isoforms as PKM2 is a highly modified protein.
PKM / Pyruvate Kinase, Muscle Antibody (Internal)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745414-100
Catalog Number: LS-C745414
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- Description
- Pyruvate Kinase, Muscle antibody LS-C745414 is an unconjugated rabbit polyclonal antibody to human Pyruvate Kinase, Muscle (PKM) (Internal). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Amphibian (Other), Rabbit
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- p58 | PK3 | PKM | PKM2 | Pyruvate Kinase | Pyruvate kinase 2/3 | Pyruvate kinase, m1 | Pyruvate kinase, muscle | TCB | Tumor M2-PK | OIP-3 | OIP3 | Opa-interacting protein 3 | CTHBP | PK, muscle type | PK2 | Pyruvate kinase isozymes M1/M2 | Pyruvate kinase muscle isozyme | THBP1
- Recommended Dilution: ELISA
- 5 ug/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P14618
- NCBI GenBank
- NM_002654 | NP_002645.3
- SwissProt
- P14618
Target
- Target
- Human PKM / Pyruvate Kinase, Muscle
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein in African frog and rat based on 100% homology and rabbit and mouse based on 93.3% homology for the immunogen sequence.
- Gene Name
- PKM
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl