EKLF was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C:0.5 mg Raw264.7 Whole Cell Lysate. 4 uL anti-EKLF rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-EKLF rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa.
PIK3R1 / p85 Alpha Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C814980-100
Catalog Number: LS-C814980
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- P85 Alpha antibody LS-C814980 is an unconjugated rabbit polyclonal antibody to human p85 Alpha (PIK3R1). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- p85 | p85 Alpha | p85-ALPHA | PI3K regulatory subunit alpha | PI3-kinase subunit p85-alpha | GRB1 | PIK3R1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P27986
- NCBI GenBank
- M61906
- SwissProt
- P27986
Target
- Target
- Human PIK3R1 / p85 Alpha
- Antigen Species
- Human
- Epitope
- Human PIK3R1
- Gene Name
- PIK3R1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human PIK3R1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS