Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody. Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.
PIK3C3 / VPS34 Antibody (Biotin)
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
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- Description
- VPS34 antibody LS-C775759 is a biotin-conjugated rabbit polyclonal antibody to VPS34 (PIK3C3) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HVps34 | Phosphatidylinositol 3-kinase | VPS34 | PI3-kinase type 3 | PI3K type 3 | PIK3C3 | PtdIns-3-kinase type 3
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q8NEB9
- NCBI GenBank
- NM_002647 | NP_002638.2
- SwissProt
- Q8NEB9
Target
- Target
- Human PIK3C3 / VPS34
- Antigen Species
- Human
- Epitope
- Detects ~101.5 kDa.
- Gene Name
- PIK3C3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human PI 3 Kinase Class 3
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS