Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.