IHC analysis of Elafin using anti-Elafin antibody. Elafin was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-Elafin antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
PI3 / Elafin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756613-100
Catalog Number: LS-C756613
For bulk orders or custom formulations:
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- Description
- Elafin antibody LS-C756613 is an unconjugated mouse monoclonal antibody to human Elafin (PI3). Validated for ELISA and IHC.
- Application
- ELISA, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Cementoin | Elafin | Elastase-specific inhibitor | ESI | PI-3 | PI3 | Protease inhibitor WAP3 | Trappin-2 | Peptidase inhibitor 3 | Protease inhibitor 3 | SKALP | WAP3 | WFDC14 | Pre-elafin
- Recommended Dilution: ELISA
- 1 - 5 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- UniProt Number
- P19957
- NCBI GenBank
- NM_002638 | NP_002629.1
- SwissProt
- P19957
Target
- Target
- Human PI3 / Elafin
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- PI3
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl