Fluorescent image of U251 cell stained with PHOX2B Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). PHOX2B immunoreactivity is localized to Nucleus significantly.

PHOX2B Antibody (aa104-130)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Western Blot
Reactivity:	Mouse
Format:	Liquid

$401.00each

Catalog Number: LS-C163688-400

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: PHOX2B antibody LS-C163688 is an unconjugated rabbit polyclonal antibody to PHOX2B (aa104-130) from human. It is reactive with human and mouse. Validated for IF and WB.
Application: IF, WB
Reactivity: Ms
Predicted Reactivity: Human, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: PHOX2B homeodomain protein | PMX2B | Neuroblastoma Phox | Paired-like homeobox 2b | PHOX2B | NBLST2 | NBPhox | Paired mesoderm homeobox 2b
Recommended Dilution: IF/ICC: 1:10 - 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: Q99453
NCBI GenBank: NM_003924 | NP_003915.2
SwissProt: Q99453

Target

Target: Human PHOX2B
Antigen Species: Human
Epitope: This PHOX2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PHOX2B.
Gene Name: PHOX2B

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen Type: Peptide

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS