Phosphotyrosine Antibody (clone PY20, HRP)

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Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay
Reactivity:	Other
Format:	Liquid

$573.00each

Catalog Number: LS-C57996-0.5

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Description: Phosphotyrosine antibody LS-C57996 is an HRP-conjugated mouse monoclonal antibody to synthetic Phosphotyrosine. Validated for ELISA.
Application: ELISA
Reactivity: Other
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 1:1000 - 1:4000

Target: Synthetic Phosphotyrosine
Antigen Species: Other
Epitope: Recognizes phosphotyrosine, enabling the detection, characterization and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyze the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS