LSBio
Phosphothreonine Antibody (clone 18F6)

Anti-Phosphothreonine Antibody - ELISA. ELISA results of Mab anti-phosphothreonine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1 ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) and TMB substrate were used for detection.

Phosphothreonine Antibody (clone 18F6)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Western Blot
Format:Liquid
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$586.00each
Catalog Number: LS-C60053-100

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Overview

Description
Phosphothreonine antibody LS-C60053 is an unconjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA and WB.
Application
ELISA, FC, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:4000 - 1:20000
Recommended Dilution: WB
1:500 - 1:2000

Target

Target
Phosphothreonine
Epitope
This product is clarified ascites produced in balb/c mice using clone 18F6. Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
18F6
Isotype
IgG1
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Ascites, 0.01% Sodium Azide
Volume
100 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.