LSBio
Phosphoenolpyruvate carboxylase Antibody (Lyophilized)

Western Blot of Rabbit Anti-Phospho Enol Pyruvate (PEP) Carboxylase antibody. Lane 1: Phospho Enol Pyruvate (PEP) Carboxylase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Phospho Enol Pyruvate (PEP) Carboxylase primary antibody at 1:1,000 overnight at 4 degrees C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 100 kDa, 100 kDa for Phospho Enol Pyruvate (PEP) Carboxylase. Other band(s): None.

Phosphoenolpyruvate carboxylase Antibody (Lyophilized)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Plant
Format:Lyophilized
See all Maize Phosphoenolpyruvate carboxylase antibodies →
$579.00each
Catalog Number: LS-C209229-100

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Overview

Description
Phosphoenolpyruvate carboxylase antibody LS-C209229 is an unconjugated rabbit polyclonal antibody to maize Phosphoenolpyruvate carboxylase. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Plnt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:10000 - 1:40000
Recommended Dilution: WB
1:1000 - 1:4000

Target

Target
Maize Phosphoenolpyruvate carboxylase
Antigen Species
Plant
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize]. Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Phospho Enol Pyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase.

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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