LSBio
PHOA Antibody (Lyophilized)

Western Blot of Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT.

PHOA Antibody (Lyophilized)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:E. coli
Format:Lyophilized
See all E. coli PHOA antibodies →
$558.00each
Catalog Number: LS-C209633-100

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Overview

Description
PHOA antibody LS-C209633 is an unconjugated rabbit polyclonal antibody to e. coli PHOA. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
E.col
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Bacterial alkaline phosphatase | JW0374 | PHOA | PsiA | ECK0378
Recommended Dilution: ELISA
1:4000 - 1:20000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P00634
SwissProt
P00634

Target

Target
E. coli PHOA
Antigen Species
E. coli
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Gene Name
phoA

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Alkaline Phosphatase [E.coli]

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.